Mucolytic activity evaluation on a fully differentiated human airway epithelium (MucilAir™)
The aim of this assay is to perform a short-term (1 and 4 days) evaluation of the effect of compounds on mucociliary clearance, cilia beating frequency and mucus secretion on a fully differentiated human airway epithelium (MucilAir™).
The end-points listed below are quantified:
Effect on mucolytic activity
Cilia beating monitoring: Evaluation of cilia beating frequency (quantitative)
Mucociliary clearance analysis (quantitative)
Mucin Secretion: ELLA assay (quantitative)
Effect of isoproterenol and apical saline solution on MucilAir™-Normal after 24h exposure. Isoproterenol significantly increases both cilia beating frequency and mucociliary clearance.
Control 1: Global markers of Bio barrier Toxicity
- Tissue integrity monitoring: TEER measurement (quantitative)
- Cytotoxicity monitoring: LDH Release (quantitative)
- Morphological modifications: Visual inspection (qualitative)
Control 2: Pro – inflammatory effect²
- Inflammatory marker: Measurement of Il-8 release (Elisa, quantitative)
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