The aim of this assay is to evaluate and rank the anti-inflammatory effect of compounds through systemic or airborne delivery.
The inflamed condition of the airway epithelium is mimicked by stimulating MucilAir with known pro-inflammatory mediators such as LPS or 10% fetal bovine serum.
The anti-inflammatory effect is monitored by IL-8 release: First, MucilAir is incubated with LPS or TNF-α or FBS 10% in culture medium. Test compound is next added to the apical (to mimic airborne dlivery) or at the basolateral side (to mimic systemic exposure) of MucilAir.
Ctrl-: vehicle control (0.9% NaCl); ctrl+: 10% FBS; 25 and 100 μg/ml of Mometasone Furoate in culture medium was applied to the baso-lateral compartment.
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