Acute, long term or repeated dose testing of drugs/formulations or xenobiotics on reconstructed human airway tissues (MucilAir™)
- Rank potential bio-barrier toxicity
- Detect pro-inflammatory profile
Acute, long term or repeated dose testing of drugs or formulations on a 3D model of fully differentiated human airway epithelium (MucilAir™) will be performed. The goal of the study is to evaluate the potential toxicity and inflammatory effects of reference compounds on daily exposure.
Apical or basolateral exposures studies can be run to mimic airborne or systemic delivery respectively.
Several endpoints, listed below, are evaluated:
Global markers of Bio Barrier-Toxicity
- Tissue integrity monitoring: TEER measurement (quantitative)
- Cell viability monitoring: Resazurin test (quantitative)
- Cytotoxicity monitoring: LDH Release (quantitative)
- Morphological modifications: Apical pictures recording (qualitative)
- Cilia beating monitoring: Evaluation of cilia beating frequency (quantitative)
Example of a 90 days repeated dose exposure study on MucilAir™. 6 hours per day exposure to formaldehyde for a period of 90 days.
Every day, tissue integrity (TEER) was measured (N=3) then epithelia were reused for the next exposure.
Pro – inflammatory effect
Measurement of cytokines/chemokines or metalloproteinases: Il-8, Il-6, etc.. release
Figure: Example of Repeated Dose Exposure Study. Pro-inflammatory profiling is detected by measuring IL-8 release. Product X was applied twice per day for a period of 20 Days. Interestingly, reversible vs irreversible effects can be pointed out.
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