MucilAir™: A unique 3D Human Airway Epithelia reconstituted in vitro
General features of MucilAir™
- Reconstituted using human primary cells
- Since 2006 we produce MucilAir™ with high standards of quality and reproducibility
- Remains fully differentiated and functional for over one year in culture
- Ready and easy to use
MucilAir™ is available from:
- Single donor or pool of donors available in nasal healthy version
- Different anatomical sites (Nasal, Tracheal or Bronchial)
- Patients with several pathologies:
- COPD (Chronic Obstructive Pulmonary Disease)
- Cystic Fibrosis (Several mutations available)
- Allergic Rhinitis
Schematic representation of MucilAir™
- Air-Liquid interface
- Production of mucus
- Active ions transport
- Tight junctions
- Metabolic activity
- Cytokines, chemokines,
- Serum free medium
- Worldwide shipping
- One year shelf life
Morphology and Histology of MucilAir™:
- Radial cut of MucilAir™: C=Ciliated cells; M=Mucus cells; B=Basal cells.
- Cross section of cilia, the microtubules’ organization is visible.
- Tight junction between two cells of MucilAir™.
- Beginning of ciliogenesis.
Ciliogenesis of MucilAir™:
A time course of cilia formation monitored by anti-β tubulin immunostaining over 60 days.
Co-staining of actin (phalloidin) and tubulin (anti-β tubulin antibody) on a 30 days old MucilAir epithelium.
After 45 days the epithelia are fully differentiated and ciliogenesis is complete, the result can be observed by Electron Microscopy:
- MucilAir™ expresses specific respiratory epithelia CYPs in a stable manner over one year
- 2A13, 2B6, 2F1 are highly expressed
- 2A6 and 2C8-2 respectively have moderate and low expression levels
- For more information on more CYPs, please see here
Videomicroscopy of MucilAir™:
Videomicroscopy of a MucilAir™ epithelium reconstituted from a Healthy donor. Coal dusts were applied on top of the epithelium, we can see an organization of the muco-ciliary clearance.
Videomicroscopy of a MuciAir™ epithelium reconstituted from a patient suffering Cystic Fibrosis. Coal dusts were applied on top of the epithelium but no muco-ciliary clearance is visible.